Whole-genome FFPE sequencing

Powered by SeqPlus

Get Better FFPE Sequencing


While FFPE has long been the standard for storing tumor samples, using this rich resource for Whole-Genome Sequencing has been limited by the poor quality of FFPE-derived DNA.

At WuXi NextCODE, we have developed our SeqPlus service for Whole-Genome Sequencing of Formalin-fixed, Paraffin-embedded (FFPE) samples to generate reliable, high-quality data. Our method produces sequence alignments with 98% of the genome covered at a depth of 20X. This process produces results comparable to fresh frozen-derived data, enabling quality mutation and copy number variation analyses.

A novel DNA extraction method using FFPE samples

Longer fragments extracted with the WuXi NextCODE SeqPlus service offering

A novel DNA extraction method using FFPE samples
Whole-genome coverage comparison

Whole-genome coverage comparison

Greater coverage than traditional methods

Our Offerings

SeqPlus Lab

Whole Genome Sequencing of your FFPE sample with a FASTQ file returned. Pricing based on number of individual samples, as few as 1. Customer conducts their own analysis.

SeqPlus Secondary

Sequencing of your FFPE sample as well as aligned data, output is a BAM file. Pricing based on number of individual samples, as few as 1. Customer conducts their own analysis.

SeqPlus Interpretation

Sequencing of 10 or 20 slides, with full analysis. A report will be provided with sample and sequencing QC metrics including DNA quality, coverage, PCR duplicates, numbers of mutations, SNP and indel counts, and sample profile including mutational signatures, and other sample/cohort specific information.

In Depth Webinar

Watch a webinar on how our FFPE Sequencing works: “Exploring high-quality whole-genome sequencing of FFPE samples from a large cancer cohort”

Get Better FFPE Sequencing


Other Products & Services


GeneCODE

GeneCODE

Learn More