While FFPE has long been the standard for storing tumor samples, using this rich resource for Whole-Genome Sequencing has been limited by the poor quality of FFPE-derived DNA.
At WuXi NextCODE, we have developed our SeqPlus service for Whole-Genome Sequencing of Formalin-fixed, Paraffin-embedded (FFPE) samples to generate reliable, high-quality data. Our method produces sequence alignments with 98% of the genome covered at a depth of 20X. This process produces results comparable to fresh frozen-derived data, enabling quality mutation and copy number variation analyses.
Longer fragments extracted with the WuXi NextCODE SeqPlus service offering
Watch a webinar on how our FFPE Sequencing works: “Exploring high-quality whole-genome sequencing of FFPE samples from a large cancer cohort”