SeqPlus: DNA from FFPE

New data from old samples

A breakthrough process to extract DNA from FFPE samples

SeqPlus: DNA from FFPE

98% genome coverage from FFPE samples

Unlock new insights from tumor samples with 98% sequence alignments covered at a depth of 20x. This process produces reliable, high-quality results for whole genome, exome, or multi-omic analysis comparable to fresh frozen-derived data, enabling quality mutation and copy number variation analyses.

Unlock novel insights, develop more targeted therapies, and speed time to value with SeqPlus.

Results comparable to fresh frozen

Generates sequence alignments covering 98% of the genome at a depth of 20x or greater, when sequenced at a depth of 70x for whole genome sequencing.

WGS from FFPE samples!

Unlike traditional FFPE sequencing processes, ours can isolate enough high-quality DNA to achieve good coverage across whole genome or whole exome sequencing.

CNV analysis

Allows for high quality mutation and copy number variation analysis.

Add sequencing to your current work flow

In addition to pathology, you can now add whole genome and whole exome sequencing to your work flow.

Proven using 15 year old archival tissue

Our assay works with archival tissue biobank samples as old as 15 years, routine clinical processing of samples for clinical trial recruitment, and tissue sections as part of normal clinical diagnostic workflows.

Salvage your degraded samples

Our proprietary FFPE process can work on specimens with degraded DNA, cross-linked nucleic acids, and low quantities of material.

Related items


White Paper: A Breakthrough Process for FFPE Samples

Isolating sufficient quality DNA from samples is a major challenge in genomic research. Setbacks can raise costs, cause delays, and lead to failed studies. A new process from WuXi NextCODE, SeqPlus, offers time and cost savings with excellent results. SeqPlus greatly improves DNA extraction from formalin-fixed, paraffin-embedded samples.

Learn More
DNA strand

Exploring high-quality whole-genome sequencing of FFPE samples from a large cancer cohort

Hear Shannon Bailey present the results from a large cancer sequencing project where WuXi NextCODE's method was used to generate high-quality WGS data from FFPE tumor-normal sample pairs.

Learn More

Taking on Cancer: Breakthroughs in DNA Sequencing Using TCGA & AI

Our team has developed the WuXi NextCODE SeqPlus FFPE extraction method, which provides substantially improved coverage compared to traditional methods and even approximates the results obtained with fresh frozen samples at 10X depth, with similar numbers of heterozygous and homozygous calls.

Learn More