A Breakthrough Process for FFPE Tissue Samples

Isolating sufficient quality DNA from samples is a major challenge in genomic research. Setbacks can raise costs, cause delays, and lead to failed studies. A new process from WuXi NextCODE, SeqPlus, offers time and cost savings with excellent results. SeqPlus greatly improves DNA extraction from formalin-fixed, paraffin-embedded samples.

Finding good samples is key for successful genomic studies. But researchers face a major dilemma:Formalin-fixed, paraffin-embedded (FFPE) tissue samples are plentiful but not easy to use. It is difficult to isolate high quality DNA from them. Therefore, NGS sequencing of such samples requires very high levels of attempted sequence coverage to compensate for uneven coverage. Until our new method wasmdeveloped, it was impossible to achieve high enough levels of coverage for whole genome sequenced samples to be usable. Fresh frozen samples, meanwhile, provide better DNA yields but are expensive to collect, much less plentiful, and harder to store. Researchers can spend many hours deciding which types of samples to use, only to find the DNA extracted is of too poor quality to generate useful results.
There is finally an answer to this problem. WuXi NextCODE has developed a proprietary, breakthrough nucleic acid extraction methodology, SeqPlus, that delivers maximum value for FFPE sequencing with coverage comparable to that achieved with fresh frozen samples. This novel extraction method avoids the traditional techniques, which break up proteins at high temperatures with vortexing and lead to smaller DNA fragments for analysis. The gentler SeqPlus method more efficiently extracts fragments of
higher molecular weight than traditional methods.


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